Enzymatic Synthesis and Structural Confirmation of Novel Oligosaccharide, D-Fructofuranose-linked Chitin Oligosaccharide

抄録

Utilizing transglycosylation reaction catalyzed by β-N-acetylhexosaminidase of Stenotrophomonas maltophilia, β-D-fructofuranosyl-(2↔1)-α-N, N´diacetylchitobioside (GlcNAc₂-Fru) was synthesized from N-acetylsucrosamine and N, N´-diacetylchitobiose (GlcNAc₂), and β-D-fructofuranosyl-(2↔1)-α-N, N´, N´´-triacetylchitotrioside (GlcNAc₃-Fru) was synthesized from GlcNAc₂-Fru and GlcNAc₂. Through purification by charcoal column chromatography, pure GlcNAc₂-Fru and GlcNAc₃-Fru were obtained in molar yields of 33.0 % and 11.7 % from GlcNAc₂, respectively. The structures of these oligosaccharides were confirmed by comparing instrumental analysis data of fragments obtained by enzymatic hydrolysis and acid hydrolysis of them with known data of these fragments.