Large-scale Preparation of 1,2-β-Glucan Using 1,2-β-Oligoglucan Phosphorylase

Koichi Abe; Masahiro Nakajima; Motomitsu Kitaoka; Hiroyuki Toyoizumi; Yuta Takahashi; Naohisa Sugimoto; Hiroyuki Nakai; Hayao Taguchi

doi:10.5458/jag.jag.JAG-2014_011

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Large-scale Preparation of 1,2-β-Glucan Using 1,2-β-Oligoglucan Phosphorylase

Koichi Abe, Masahiro Nakajima, Motomitsu Kitaoka, Hiroyuki Toyoizumi, Yuta Takahashi, Naohisa Sugimoto, Hiroyuki Nakai, Hayao Taguchi

著者情報

キーワード: 1,2-β-glucan, 1,2-β-oligoglucan phosphorylase, sucrose phosphorylase, glycoside hydrolase family 94

ジャーナルフリー早期公開
電子付録

論文ID: jag.JAG-2014_011

DOI https://doi.org/10.5458/jag.jag.JAG-2014_011

この記事には本公開記事があります。

The final version of this article is now available: Vol. 62 (2015), No. 2 pp. 47-52

詳細

抄録

1,2-β-Glucan was produced enzymatically from 1.0 M sucrose and 0.5 M glucose by the combination of sucrose phosphorylase and 1,2-β-oligoglucan phosphorylase in the presence of 100 mM inorganic phosphate. Accumulation of 1,2-β-glucan in 2 L of the reaction mixture reached over 800 mM (glucose equivalent). Sucrose, glucose and fructose were removed after the reaction by yeast treatment. 1,2-β-Glucan was precipitated with ethanol to obtain 167 g of 1,2-β-glucan from 1 L of the reaction mixture.

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