Abstract
A model experiment was performed to simulate the process of precipitation of starch molecules in plastids. This suggested the presence of a soluble form of ADP -glucose α-1, 4-glucan-α-4-glucosyltransferase (starch synthetase) in spinach chloroplasts . The enzyme was purified in order to use it as chain elongator in a system for starch granule formation in vitro, which is to be constructed by well-characterized enzymes. It was found that the spinach enzyme catalyzes the formation of a long chain of α-1, 4-linked glucose units.