Abstract
Cycloamylose glucanotransferase (CGT, EC 2.4.1.19) from Bacillus stearothermophilus was confirmed to catalyse the reaction for a substrate, maltose. When this reaction was carried out in the presence of isomaltose, a trisaccharide (X3) was primarily produced. Based on glucoamylase digestion and NMR analysis, X3 was concluded to be isopanose. The reaction is probably one of the most useful techniques for the production of Isopanose. In the presence of cellobiose/lactose, the CGT reaction for maltose was found to result in new products trisaccharide and tetrasaccharide having α- and δ-glycosidic linkages. This reaction system will be used to produce some oligo-sugarchains ("tousa").
