Abstract
We performed a kinetic study to estimate the action mode of two α-amylases, TVA I and TVA II, on glucosyl cyclodextrins. The enzyme genes from Thermoactinomyces vulgaris R-47 were individually cloned and expressed in Escherichia coli, and the enzymes were prepared from the transformants. TVA I hydrolyzed glucosyl-γ-cyclodextrin well, whereas TVA II hydrolyzed glucosyl-α- and -β-cyclodextrins. TVA I and TVA II hydrolyzed glucosyl cyclodextrins to produce glucose, maltose, panose, and 62-α-glucosylmaltotriose.
