Purification and Some Properties of an Extracellular Oligo-1, 6 Glucosidase from Bacillus stearotheymaphilus SA0301

Abstract

Bacillus stearothermophilus designated strain SA0301 produces extracellular oligo-1, 6-glucosidases during the stationary phase of growth. The enzyme was purified to an electrophoretically homogeneous form. Its molecular mass was estimated to be 63 kDa by SDS-PAGE, and its N-terminal amino acid sequence was MERKWWKEAVVYQIYP-. The enzyme was shown to hydrolyze isomaltose, isomaltotriose, and panose, but not trehalose, sucrose, or maltose.