Synthesis of N-Acetylglucosaminyl-Maltosyl-Cyclodextrin by Lysozyme

Abstract

Hetero branched CDs linked by N-acetylglucosamine were synthesized from a mixture of N-acetylchitooligosaccharides and branched CDs by transglycosylation of lysozyme. A transfer product from N-acetylchitooligosaccharides and maltosyl-βCD was identified as 6-O-α-(3²-O-β-b-N-acetylglucosaminyl-) maltosylβCD. The optimum pH at 60°C and the optimum temperature at pH. 4.5 to produce N-Getylglucosaminyl-maltosyl-RCD by lysozyme were pH 3.5-6.0 and 60-70°C, respectively. Lysq zyme was unable to link N-acetylglucosamine to mannosyl-CD, galactosyl-CD and directly to CD rings.