Abstract
For these 20 years our research groups have investigated the reaction mechanism of glycoenzymes as follows; (1) glucoamylases from Rhizopus niveus and delemar, (2) CGTase from Bacillus stearothermophilus, (3) BLA, (4) BSA, (5) TAA, (6) xylose isomerase from Streptomyces phaeochromogenus, (7) β-glucosidase from Aspergillus niger, (8) β-galactosidase from yeast, (9) β-glucanase from yeast, (10) β-glucocerebrosidase from animal (wagyu). A lot of experimental results have been accumulated in this research era. However, all the reactions were carried out in aqueous solutions, where the reaction system is three dimensions. This is an important point in this discussion. As described in textbooks, for example The Cell, the rate in a reaction system of two dimensions is much larger (around 15 fold) than that of three dimensions. Thus we are very interested in the reaction system of two dimensions, which reaction field in a biological system is the membrane. As a consequence, research on the enzymes and proteins in membrans will be critically important for biological science and technology. Here, based on the research into glycoenzymes, we intended to develop a new research field, that is, cellular macromolecules.
