Search for pterin-binding protein from Euglena

抄録

BIAcore analysis with a biopterin (BP)-bound sensor chip could be a powerful tool for the search of pterin-binding proteins. Although preparation of the sensor chip is crucial, a conventional amine coupling procedure is not applicable for the ligand such as BP that has no primary amino group. In this study, we describe the method to introduce the primary amino group onto BP molecule efficiently.