Screening of Epithelial Cell-Adhesive Peptides from Fibronectin Loop Region and Its Cell Specificity

抄録

Using a peptide array-based cell system, screening of cell adhesive peptides has been investigated against two epithelial-type cells, such as mouse lung epithelial cell (mLEC) and mouse glomerular epithelial cell (mGEC), and one endothelial cell such as human umbilical vein endothelial cell (HUVEC). When 63 pentamer peptide sequences of human fibronectin type III domain were synthesized on the cellulose membrane assayed, GSKST (No. 124) and GLKPG (No. 126) showed high adhesion activity against all three type cells. The adhesion pattern of mGEC using the library was different from mLEC. Eight adhesive peptides were found for mGEC as peptides with higher adhesion activity compared with HUVEC. It was found that the adhesion pattern of mLEC was similar to that of human mesenchymal stem cell (MSC), not HUVEC. The results suggest that a tissue specific adhesion peptides can be designed, which has the potential for the development of a tissue specific molecular tag.