Abstract
It was previously reported that free iron was released from rat red blood cells in both an in vitro study with a metabolite, mono-n-butylphthalate (MBP), of di-n-butylphthalate (DBP) and an in vivo study with oral DBP, inducing rat testicular atrophy in hypoxic conditions due to MBP-induced hemoglobin deprivation. This study investigated whether mono-n-alkylphthalates (alkyl-carbon C4-C6 ; MAP), which result in rat testicular damage, induce iron release from hemoglobin by incubation with rat red blood cells. Iron release was observed with C3-C6 MAP incubation at lower doses and with C1 and C2 MAP incubation at higher doses, but not with C7, C8, and C4(6) MAP.
