Assay-guided Informatory Screening Method for Antiplatelet Effect of Adenosine Isolated from Malbranchea filamentosa IFM 41300: Inhibitory Behaviors of Adenosine in Different Solvents

Abstract

A particle-counting aggregometer employing laser-light scattering was used in systematic manners to screen and to detect inhibitor(s) of platelet aggregation from the extract of Malbranchea filamentosa (M. filamentosa) IFM41300. The inhibitor was determined as adenosine on the basis of the ¹³C- and ¹H-NMR spectral data. Although adenosine was previously reported as an inhibitor of platelet aggregation, we isolated the compound from the fungus for the first time. Because the method was able to provide us with information on the developmental formation of platelet aggregates in different sizes with incubation time, we further elaborated the inhibitory behaviors of adenosine, as an example, at varied concentrations in different solvents such as dimethyl sulfoxide (DMSO) and saline. We found that DMSO could facilitate to dissolve less water soluble materials from herbs and fungi by using the present assay method.