Abstract
Separation analysis of nine kinds of antioxidants and quantitative determination of BHA were investigated by means of silica gel thin-layer chromatography. Benzene-ether (1 : 2) and chloroform-ether (1 : 3) were found as suitable solvent systems for two dimensional chromatography, and the minimum amount for identification was 1 μg per ml. For determination of BHA, 2, 6-dichloroquinone 4-chlorimide were used as a color reagent and the colored spot was scraped off from the plate, extracted with ether and determined spectrophotometrically.
