Concentration of Vitamin K₁ in Rat Liver after Administration of Vitamin K₁

Abstract

The content of vitamin in K₁ in rat liver was determined at various time intervals after the intraperitoneal injection of Vitamin K₁. Seperation and purification of Vitamin K₁ from liver extracts were carried out by alumina column chromatography follwed by thin layer chromatography. The spot on the thin layer plate was extracted and determined fluorometrically as reported previously. The amount of vitamin K₁ in liver reached maximum within 3-6 hours after injection and became undetectable in 12-18 hours later. Any characteristic accumulation of this fat soluble vitamin was not observed in liver.