Studies on Identification of Thiamine Related Compounds and Thiamine Decomposed Compounds by the Paper Electrophoresis

Abstract

Thiamine related compounds (five samples) and thiamine decomposed products (seven samples) could be identified by the paper electrophoresis with 5 N acetic acid as the electrolyte. From further investigation of electrolyte, it was found that borax (1%) was effective for identification between allithiamine and DBT, and that formic acid (5 N) was effective for identification beetween TDS and thiazole·pyrimidine compound, and also between thiochrome and thiazolone compound. Applying this analytical methods, it was observed that alkali degradation process of thiamine to thiazolone compound was influenced by various pH, and that hydrolysis was occured exclusively in the condition of heating aqueous thiamine solution at 130°.