Studies on the Quality of Pharmaceutical Preparations. VI. Determination of Aescin in Aesculus Hippocastanum L. Seed Extract Preparation by Fluorometry

Abstract

A new method is described for the quantitative determination of aescin in the seed extract of Aesculus Hippocastanum L. using a thin-layer chromatography and fluorometry. A sample was separated on a thin-layer of Wakogel B-5, developed by water-saturated BuOH : AcOH (6 : 1), and a spot of aescin (Rf=0.35) was detected by irradiation of an ultraviolet lamp (main wave length 3650 Å) after spraying the KMnO₄-H₂SO₄ reagent. The fluorescent spot of aescin was scraped off completely and extracted with 85% H₂SO₄. The solution extracted was excited at4 12nm and measured at 454nm. Linearity between the amount of aescin and fluorescence intensity was recognized between 25 and 100 mg/10 ml.