Abstract
Gas chromatographic determination of 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (furyl furamide) in food was established as follows. A mixture of more than 1 μg of furyl furamide, 10 μl of hepta fluorobutyric anhydride (HFB), and 0.5 ml of acetone in a 25 ml light-resistant glass test tube is allowed to stand for 5 min at room temperature. After removal of unreacted HFB with a capillary tube under a reduced pressure, acetone is added exactly to the volume indicated. The sample so obtained is subjected to gas chromatography. The lower limit of the sample size of furyl furamide for identification was 1 ng. The method presented here may be applied to determine furyl furamide contained in food as a fungicide.
