Abstract
The application of radioimmunoassay for using anti-phenobarbital antibody is described. 1) Barbiturate was identified by this method using 0.1 ml of serum drawn from a person in coma after an attempted suicide. 2) Phenobarbital or its metabolites excreted in the urine of a human receiving phenobarbital in a dose of 40 mg/44 kg can be detected for almost 3 weeks by this method. 3) Unknown minor metabolites were found from the urine of rat receiving phenobarbital by this method combined with thin-layer chromatography. These results were discussed in relation to the specificity of the antibodies.
