Abstract
The Actinomycetes strain A, isolated from the bottom sediment in the Sengari Reservoir, the largest water source of Kobe city, was cultured in the MYA (malt, yeast extract agar) medium at 25°for 10 days, and the culture with the medium was submitted to steam distillation to concentrate the volatile odoriferous substance. This distillate was systematically extracted into fractions I (neutral substance), II (phenolic substance), III (acid substance), IV (basic substance), and V (amphoteric substance). From the retention time in gas chromatography and from the result of gas chromatographymass spectrometry, geosmin and 2-methylisoborneol, the musty odor substances, and 2-phenylethanol aving aromatic (flowery) odor were isolated from fraction I, and phenol and m-cresol were identified in fraction II. A basic substance with a musty odor was isolated from fraction IV but its structure has remained unidentified.
