Studies on Environmental Contamination by Polychlorinated Naphthalenes (PCN). I. Concentration of PCN in Water Samples by Column Chromatography and Its Determination by Gas-Liquid Chromatography

Abstract

A method in which polychlorinated naphthalenes (PCN) residual at trace concentration in water are concentrated using an alkylbenzenesulfonic acid-binding polyamide column and determined by gas-liquid chromatography was investigated. The sample was submitted to the polyamide column and then PCN were eluted from the column with ethanol. After dilution of the eluate with water, PCN were extracted with hexane, and the extract was washed with concentrated sulfuric acid. After evaporation of hexane, the residue was applied to polyamide thin-layer chromatography, giving a good separation of PCN from polychlorinated biphenyls and chlorinated pesticides. The purified PCN were determined by gas-liquid chromatography (ECD). The water samples fortified with PCN at a level of 0.5 ppb were successfully determined, giving the recovery of 83.9-86.9%.