Abstract
A simple method for the determination of sucrose monopalmitate (SMP) by the use of gas chromatography is described. SMP was trimethylsilylated with a mixture of hexamethyldisilazane and trimethylchlorosilane or of N, O-bis (trimethylsilyl)-acetamide and trimethylchlorosilane in anhydrous pyridine. Trimethylsilylated SMP was introduced into a gas chromatograph equipped with a hydrogen flame ionization detector and a glass column (0.5 m×3 mm) packed with 3% OV-17 on Gaschrome Q at 260°in N2 with a flow rate of 55 ml/min. SMP was resolved from sucrose monostearate (SMS) and was determined by the use of SMS or trilaurin as an internal standard. A linear relationship between the peak height ratio of trimethylsilylated SMP to internal standard and the amount of trimethylsilylated SMP injected was obtained up to at least the amount equivalent to 25 μg of SMP. Coexistent palmitate in the sample was also determined, after treatment of the sample with the trimethylsilylating reagents mentioned above, by employing the same gas chromatograph equipped with another glass column (1.0 m×3 mm) packed with 3% OV-17 on Gaschrome Q. The recoveries of SMP and palmitate from rat plasma or lymph were satisfactory.
