Abstract
A rapid method for high-speed liquid chromatographic determination of micro amounts of estradiol or ethynyl estradiol in cosmetics was investigated. This method is based on the technique of direct dilution or solvent extraction of the samples by high speed liquid chromatography with octyl silane (LiChrosorb RP-8, 5 μ) as a stationaly phase, a mixture of acetonitrile-water as the eluent, and fluorescence spectrophotometer as a detector. The retention time of n-butyl-p-hydroxybenzoate in some creams that interfered in the determination was controlled by the addition of trioctylmethylammonium chroride to the eluent. Estradiol or ethynylestradiol in cosmetics at the level of 0.00015-0.00100% was determined by this method with a recovery rate of 93-103%.
