Abstract
1-Naphthlamine and 2-naphthylamine remaining in dyestuffs were analyzed with high accuracy and sensitivity by high-speed liquid chromatography (Hitachi 635 T Liquid Chromatograph) after tosylation of both. By elution with methanol : ethyl acetate : water (48 : 12 : 40, v/v) on a JASCOSIL SC-01, tosylated 1- and 2-naphthylamines were completely separated in only 20 min without gradient elution technique. The common and accurate detection of both tosylated naphthylamines was possible near 275 nm using an ultraviolet monitor. With this method, the amount of tosylated naphthylamines was measured within 1% error over the range of 0.02-6 μg, and the limit of detection was 0.02 μg.
