High-Speed Liquid Chromatographic Determination of o-Phenylphenol (OPP) in Citrus Fruits

Abstract

A method for high-speed liquid chromatographic determination of o-phenylphenol in citrus fruits was investigated. Determination was carried out successfully by using p-tert-amylphenol as an internal standard. o-Phenylphenol and the internal standard were steam-distilled from citrus fruit samples, and extracted with ether from the resulting distillate after washing with carbon tetrachloride. The recovery of o-phenylphenol was 93.8% (σ rel. 1.5%).