Abstract
Quantitative screening method for bicozamycin in chicken egg and meat by fluorescence densitometry with high performance thin layer chromatography (HPTLC) was developed. Bicozamycin was extracted with 2% phosphotungstic acid from chicken egg and meat. The extract was cleaned up by a XAD-4 resin column with 60% methanol as an eluent. The eluate was evaporated to dryness, and the residue was dissolved in methanol and acetone. The solution was spotted on a silica gel HPTLC plate. After development, the plate was dipped in 2% sulfuric acid-ether solution and heated. The spots of bicozamycin gave blue fluorescence under long wavelength ultraviolet (UV) light. The fluorescence intensity was determined by densitometry. The calibration curve was rectilinear in the range from 3 ng to 30 ng. The average recoveries of bicozamycin added to chicken egg and meat were 91.8% and 91.4%, respectively. The present method will be applicable to determine bicozamycin with the detection limit of 0.2μg/g.
