Abstract
Hydrogen sulfide (H2S) in fluid and tissue samples of rat or human was determined by gas-liquid chromatography (GLC) with a flame photometric detector (FPD). By using a stationary phase of 25% TCEP on Chromosorb W (AW-DMCS), suitable conditions were obtained and the H2S peak appeared as a retention time of 0.7 min. The minimum detectable concentration by this method was 1 μg/ml. For the determination of fluid sample, to 1 ml (or 1 g) of sample (In the case of tissue samples : 1 g of sample was homogenized within 4 ml of cold water. After centrifugation, 1 ml of supernatant was used in the same way as fluid sample.) was added 5 ml of acetone and 0.5 ml of 20% hydrochloric acid. After mixing for 2 min and centrifuging for 5 min at 1000 g, the supernatant was used for GLC-FPD. The recoveries of H2S from fluid and tissue samples in rat or human were about from 50 to 100%. The GLC-FPD method for the determination of H2S is very useful in its simplicity, rapidity, efficiency and reproducibility.
