Detection and Quantitative Determination of Lipid Peroxidation in Living Bodies : Thiobarbituric Acid Test

Abstract

Detection and quantitative determination of the components : i) radical species, ii) hydroperoxides, iii) secondary aldehydes, hydrocarbons and fatty acids, and iv) reaction products between proteins, which are generated during lipid peroxidation, are reviewed. Determination of plural classes of components is required for the accurate monitoring of lipid peroxidation. Among the many known assay methods, thiobarbituric acid test can be properly used for the monitoring of lipid peroxidation. The principles of the test, which has been recently clarified, is reviewed in detail.