Abstract
Analysis of Trp-P-2 in municipal sewage water and investigation on its behavior during activated sludge treatment of Trp-P-2 was carried out. Prior to HPLC, we established the most suitable pretreatment-condition for soluble and adsorptive Trp-P-2 in the sewage water. Determination of soluble and adsorptive Trp-P-2 in the sewage water showed that Trp-P-2 exists in the sewage water and adsorptive form is too much more than soluble one. Activated sludge treatment of Trp-P-2 demonstrated that the most of Trp-P-2 adsorbs to activated sludge at an earlier period after the treatment of Trp-P-2. It is considered that the release of Trp-P-2 from activated sludge was recognized by sonication, alkalization or ethyl acetate extraction. Indirect frameshift mutagenicity of treated water during activated sludge treatment of Trp-P-2 showed a decrease with treatment time.
