Production of Active Oxygen Species and Lipid Peroxidation by the Reaction of Potassium Bromate and Reduced Glutathione

Abstract

Potassium bromate (KBrO₃), a food additive, has renal toxicity with the possibility of active oxygen species. It was recognized with the DTNB method that the reduced glutathione (GSH) content in the reaction mixture was correlatively decreased by the addition of KBrO₃. The peroxidation of linoleic acid micelle by the addition of GSH and KBrO₃ was measured by the ultraviolet absorption method and oxygen electrode method. Moreover, thiobarbituric acidreactive substance (TBARS) content was correlatively increased by the addition of GSH and KBrO₃ on the microsomal system in vitro. But the increase of TBARS content was inhibited by the addition of sodium azide and it was not inhibited by the addition of superoxide dismutase, catalase, mannitol, uric acid, DTPA and EHPG respectively. The addition of sodium azide correlatively inhibited the production of TBARS in the reaction mixture. These results suggested that the singlet oxygen was produced by the reaction of GSH and KBrO₃ and was related to the lipid proxidation.