Determination of Carboxy Hemoglobin by a Spectrophotometric Method with Multivariate Analysis

Abstract

The degree of carboxy hemoglobin in blood was determined by multivariate analysis for the purpose of correcting an error in the low degree region by multivariate analysis. The absorbance of blood was measured at eight points, from 530 nm to 580 nm, by a spectrophotometric method, and the data were corrected using two points within the eight measured points. These corrected values were used for multivariate analysis such as cluster analysis and principal component analysis. As a result, principal component scores were obtained, and the degree of carboxy hemoglobin in blood was determined using these scores.