Abstract
Urine samples are known to foam and gelatinize solvent when shaken. The present work sought to develop a means of separating and characterizing urinary foaming material. A method was developed which can foam and gelatinize solvent. Human urine, after being filtered at pH 10, was bubbled at pH 4-5. The foam was shaken with n-hexane to gelatinize the solvent. The gelatinized solvent layer was washed with water several times, and dried on a glass plate ; dried material was then scraped off with a razor. A soft powder was obtained which was suggested to contain protein and sugar. The two moved together electrophoretically at the site of α-globulin, and are suggested to be a complex of protein and sugar (glycoprotein).
