Abstract
A clean-up method using gel permeation chromatography (GPC), and a qualitative analysis involving the electrophoresis of soluble dietary fibers (SDFs), including Polydextrose[O!R], Pinefibre[O!R], Cellace[O!R], Sunfiber[O!R] and guar gum, in soft drinks were both developed. Impurities such as sucrose, glucose, fructose, coloring agents and vitamins were clearly eliminated by GPC with Sephadex G-10, with sufficient recoveries, in soft drinks. The solutions treated with GPC were then applied to the electrophoresis with a borate buffer. SDFs showed different migration distances and were detected colorimetrically with 4-aminopyridine. The spot of Cellace[O!R] was grayish green in color, while the other spots were pale wine red color. SDFs were also determined by a high performance liquid chromatograph equipped with a refractive index detector and a carbohydrate analysis column.
