Abstract
We previously reported the efficiency of polyamide column in the concentration of polychlorodibenzo-p-dioxins (PCDDs) from environmental water samples. This study further applied the polyamide column to concentrate of the other organic compounds causing the pollution of waters : polycyclic aromatic hydrocarbons (PAHs), polychlorobiphenyls (PCBs) and chlorophenols (CPs) from their aqueous solutions. The model compounds used were : benzo [a] pyrene, anthracene and monotrichloropyrenes (PAHs) ; KC300, KC400 and KC500 (PCBs) ; and 2, 4, 6-trichlorophenol, 2, 3, 4, 6-tetrachlorophenol and pentachlorophenol (CPs). The mixture of these compounds in much higher concentrations compared to their putative environmental existence were quantitatively adsorbed to the polyamide column. The fractional elution of the test compounds from the column was attained with ethanol (PCBs and polar PAHs), dichloromethane (nonpolar PAHs) and 5% acetic acid in dichloromethane (CPs plus PCDDs), in this order. Each fraction could be analyzed by either high performance liquid chromatograph equipped with a fluorescence monitor in the case of PAHs or gas chromatograph with an electron capture detector for PCBs and CPs. The polyamide column will be useful for the analysis of trace amounts of organic pollutants in environmental waters.
