Determination of Methamphetamine in Human Urine by HPLC Using Post-Column Simon's Reaction

Abstract

An HPLC method using post-column Simon's reaction (SR-HPLC) for the determination of methamphetamine (MA) has been developed. The detection limit was 1ng, which is about 100 times more sensitive than TLC using Simon's reaction (SR-TLC). The calibration curve was linear in the range of 0.1-1000μg/ml, and the intermediate precision of within-run and between-run assays was 1.15% and 2.12%, respectively. MA in 13 urine samples was determined by SR-HPLC and GC with flame ionization detection, and these quantitative values were strongly correlated (r²=0.995). MA could be analyzed without interference peaks. The proposed method is highly selective and quantitative.