Journal of Health Science
Online ISSN : 1347-5207
Print ISSN : 1344-9702
ISSN-L : 1344-9702
Purification and Identification of Antioxidant Substances in Human-Placenta Extracts
Shin-ichi TogashiNoriko TakahashiYoshinori KuboAtsushi ShigiharaKimio HigashiyamaSatoshi WatanabeTetsuya Fukui
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Keywords: identification

2000 Volume 46 Issue 2 Pages 117-125


We attempted to purify and identify the active antioxidants in human-placenta extracts (PLx). Purification was performed successively by 40% methanol precipitation, Sephadex G-50 gel chromatography, and high-performance liquid chromatography (HPLC). The supernatant of PLx after 40% methanol precipitation possessed 85% of the original antioxidant activity. Elution of the antioxidants from gel chromatography gave mainly three peaks with approximately 98% recovery of the antioxidant activity. The antioxidant activity was then fractionated by HPLC with a recovery of approximately 90% of the activity. Three components were obtained, and based on HPLC retention times, UV, NMR, and FAB-MASS, they were identified as uracil, tyrosine, and phenylalanine.

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