Cross-Reactivity of Various Tetrahydrocannabinol Metabolites with a Monoclonal Antibody against Tetrahydrocannabinolic Acid

Kazuhito Watanabe; Midori Matsuda; Yuji Tateoka; Toshiyuki Kimura; Tamihide Matsunaga; Hiroyuki Tanaka; Yukihiro Shoyama; Ikuo Yamamoto

doi:10.1248/jhs.46.310

Kazuhito Watanabe, Midori Matsuda, Yuji Tateoka, Toshiyuki Kimura, Tamihide Matsunaga, Hiroyuki Tanaka, Yukihiro Shoyama, Ikuo Yamamoto

Author information

Kazuhito Watanabe
Midori Matsuda
Yuji Tateoka
Toshiyuki Kimura
Tamihide Matsunaga
Hiroyuki Tanaka
Yukihiro Shoyama
Ikuo Yamamoto
Author's Organization：
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University
Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University
Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University

Keywords: tetrahydrocannabinol metabolite

JOURNAL FREE ACCESS

2000 Volume 46 Issue 4 Pages 310-313

DOI https://doi.org/10.1248/jhs.46.310

Details

Abstract

A monoclonal antibody (MAb-4A4) against Δ⁹-tetrahydrocannabinolic acid (THCA) was prepared and its cross-reactivity analyzed with various cannabinoids including tetrahydrocannabinol metabolites by competitive enzyme-linked immunoassay (ELISA). Δ⁹-THC (1-100μg/ml) could be determined by the ELISA. Many metabolites of Δ⁸-THC and Δ⁹-THC reacted with the antibody, and their cross-reactivities were 44-157% of Δ⁸-THC. However, the antibody did not recognize the lipophilic compounds cholesterol, testosterone, β-carotene, androstene-3, 17-dione or an endogenous cannabinoid, anandamide.

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