抄録
A semi-quantitative laboratory bioassay method was devised for the female sex pheromone of Brachmia macroscopa. The bioassay was carried out with male moths, 5 each in a 200-ml Erlenmeyer flask, at 25°C in the light. They were kept under continuous illumination for 3 days after emergence, subsequently in the dark for 6hr, then again in the light for 30 to 60min. The number of males rapidly walking or running with wing-fanning within the first 1min after the introduction of a test sample applied on a square filter paper (1.5×1.5cm) was used as a measure of response. Mean male response was 70% to the pheromone at the concentration of 0.1 female equivalent and about 50% at the 0.01 female equivalent.
