The Pringle Maneuver Accelerates Liver Metastasis by Over-Expression of Tumor Vasculature in a Murine Model of Colon Cancer
2004 年 29 巻 1 号 p. 31-37
詳細
2004 年 29 巻 1 号 p. 31-37
Background : We traditionally use the Pringle maneuver to avoid hemorrhage during hepatectomy.However, the mechanism of metastasis in the ischemic condition is unknown.
Method : An orthotopic model of murine colon cancer in syngeneic BALB/c mice wasused. Viable CT -26 cells were implanted into the spleen of mice. Five days after tumor implantation, mice received laparotomy and the hepato-duodenal ligament was clamped for 0 or 10 minutes (Pringle maneuver). Twelve days after tumor implantation, the mice were killed and the number of hepatic metastasis was counted.
Result : Mice which received Pringle maneuver produced a greater number of hepaticmetastasis. Immunohistochemical analysis revealed that the expression of microvessel density andVEGF were higher in hepatic metastasis with the Pringle maneuver. Also, mice which received Pringle maneuver had a higher level VEGF in the serum.
Conclusion : The Pringle maneuver induces tumor vasculature and produces a large number of liver metastasis in mice.
