ヘリウムセーバーインジェクターを用いたGC-MS/MS による有機塩素系農薬測定の検討

抄録

The Helium (He) Saver injector can dramatically reduce consumption of He gas in GC-MS(/MS) analysis in comparison to a conventional split/splitless (SSL) injector using helium carrier gas. The He Saver injector was evaluated in comparison with SSL injector using organochlorine pesticides including their metabolites (10 analytes). The standard solutions (1 ng/mL and 100 ng/mL) of the analytes were analyzed 5 times each by GC-MS/MS using the two injectors. Retention times, peak shapes and peak areas of the analytes were compared between the two injectors. The retention times obtained by the He Saver injector were in good agreement with those obtained by the SSL injector. The selected reaction monitoring chromatograms from both the injectors showed no remarkable differences in peak profiles. The average peak areas using the He Saver injector were close to those obtained using the SSL injector (94-115% of the SSL injector). Although there were statistically significant differences of the peak areas in three of the ten analytes using a two-sided t-test (p<0.05), these differences were not considered to be important in a practical analysis. Peak area ratios for the analytes (qualifier ions/quantifier ions) using the He Saver injector were also close to those using the SSL injector (94- 104% of the SSL injector). Overall results indicate that the He Saver injector can be used for as an alternative for a conventional SSL injector and contribute to a reduction of helium consumption in GC-MS(/MS) analysis.