抄録
A fully automated high-performance liquid chromatography system with column-switching was evaluated to determine the concentration of zonisamide (ZNS) in human plasma. After the plasma sample was diluted to 10-times with the mobile phase, the sample (101μL) was injected into the column-switching system without pretreatment. ZNS was detected by ultraviolet absorption at 246 nm. The calibration curve of ZNS was linear and ranged from 1.57 to 50μg/mL of plasma. The coefficients of variation in the within-run (n=5) and the between-run (5 days) precisions of ZNS were below 5 %. The limit of quantification was 0.5pg/mL in 1 μL of human plasma. A good correlation was found between the data obtained by this system and the ELISA system using Markit-M Excegran (r=0.956) or the solid-phase-extraction HPLC method (r=0.985). This system for ZNS is thus considered to be a very useful measurement for therapeutic drug monitoring because of its high sensitivity, rapidity and low cost compared with other measurement systems.
