萎黄型ウイルス病およびマイコプラズマ病感染植物の直接螢光診断法(DFD法)

抄録

Thin sections (0.5-8.0 mm thick) were prepared from fresh tissues of plants infected with phloem-limited viruses (yellows-type viruses) or mycoplasma-like organisms (MLOs). When observed under a reflecting fluorescence microscope (RFM) (Type: Olympus BHS-RF-A), they distinctly showed a yellow fluorescence in their phloem tissues. Such a fluorescence was never found in those of healthy plants. By electron microscopy of the same materials, the fluorescence was proved to originate in necrotic phloem cells. From these results, the direct detection of fluorescing cells in phloem tissues under RFM is concluded to be suitable for the diagnosis of infections by phloem-limited viruses or MLOs. We named it “direct fluorescence detection method (DFD method)”.