胸水乾燥塗抹標本中に HHV-8 DNA の存在を確認しえた原発性滲出液リンパ腫の 1 例

抄録

We report herein on a case of primary effusion lymphoma (PEL). Smears of the pleural effusion demonstrated centroblast-like atypical lymphocytes. By applying the PCR process using extracted DNA from dry smear of the pleural effusion, we could detect rearrangement of immunoglobulin heavy chain gene (FR2B-JH) and the presence of human herpesvirus-8 (HHV-8) DNA (ORF26). We consider that an effusion smear, even when dry, can be a useful material for detection of monoclonal gene rearrangement and the presence of the HHV-8 gene assessed with the PCR assay, which lead to an accurate diagnosis of PEL.