藥物その他によるアレルギーにおける白血球の関与についての研究特に白血球蛋白感作カオリン凝集反応,および白血球を用いるCytotoxic Testについて

第二篇-Cytotoxic Activity Testにおける薬物の白血球傷害作用(Cytotoxicity)について

抄録

The drug allergy sera, to each sample of which the responsible drug had been added, were examined as to their cytotoxic activity by means of the dye-exclusion technique. The results obtained were as follows:
1) Some of the sample displayed hi g h cytotoxicity even without the responsible drug, a condition which suggests the presence of auto-immune anti-leucocyte antibodies in these particular sera.
2) The addition of responsible drugs markedly elevated the cytotoxicity of the drug allergy sera.
3) Fr actionation by DEAE-cellulose column chromatography revealed that the 2"globulin fraction had the highest activity among the fractions, followed by the (32-globulin which had a much weaker activity.
4) Histamine cytotoxicity was estimated in normal serum to which various amounts of crystalline histamine and normal human leucocytes had been added.
The result suggested that the cytotoxic activity of drug a llergy sera should be equivalent to that of histamine concentrations of not less than 1.0x10-14g.