藥物その他によるアレルギーにおける白血球の関与についての研究特に百血球蛋白感作カオリン凝集反応,および白血球を用いるCytotoxic Tsetについて

第三篇-白血球蛋白感作カオリン凝集反応

抄録

In order to detect complete or incomplete antibody-like activities in drug allergy sera and also in their fractions, means of detecting agglutination reaction of kaolin particles in saline or albumin solutions were designed and carried out, i. e. the kaolin particles were first coated with leucocyte proteins. Furthermore, the influence of the papainization of the leucocytes used for the coating was also studied in order to differentiate autoantibody from iso-antibody using the method of van Loghem et al. The results were as follows:
1) C omplete antibody-like activity was detected in about 80% of the drug allergy sera. Fractionation of the sera revealed that this activity was mainly associated with the r globulin and 02-globulin fractions, and only slightly with the 01-globulin fraction.
2) In albumin solution of the drug allergy sera, the activities displayed were inconsistent.
3) The influence of leucocyte papainization on the activity of the serum protein fractions was most markedly seen in the r- and 02-globulin fractions.
These results suggest the presence of a multiplicity of an tibody-like factors in the drug allergy sera, i. e. complete, incomplete, auto-, and iso-antibody-like activities.