the quality of ceroid deposited in Kupffer cells of CCI4-inhalated mouse liver by the color intensity developed with the leucomalachite green stain or zinc leuco-dye stain for ceroid (zinc leucosafranin and leucofuchsin stain). Materials and Methods: For the purpose of measuring ceroid 3-4 serial paraffin sections were prepared from each localized area at random selected from CCI4-inhalated mouse livers. Measurements were made on 10 individual Kupffer cells filled with ceroid granules on each section. Results: The spectra of absorption curves obtained indicated that a gradual increase in the color intensity developed with both the leucomalachite green and zinc leucosafranin stain in the Kupffer cell ceroid has a tendency to run parallel with the length of the experimental period of time until 105 times of CCI4 inhalation carried out once on alternate days, the leucofuchsin stain being less effective. The above findings suggest a gradual increase in pigment maturity with the la pse of the experimental period. In order to obtain more morphological evidence, the present authors could compare with success the above absorption spectra with a diagram gained by means of dipicting “multilayer” absorption curves in which the quality of ceroid deposited in Kupffer cells is illustrated as an image of a hill, leaving an area of a nucleus blanks.
