抄録
Acanthamoeba is widely distributed in various habitats such as soil, water and can cause fatal infections in humans. With the global incidence of Acanthamoeba infections increasing and no specific treatment currently available, research into novel anti-Acanthamoeba agents remains essential. Similarly, cancer cases are also rising worldwide, and effective treatments continue to be a major challenge. In this context, the present study aimed to investigate the anti-Acanthamoeba and anticancer activities of marine Actinomycetes isolated from Türkiye. Fifteen Actinomycetes cell-free supernatants were prepared and used in this study. To evaluate anti-Acanthamoeba effects, Acanthamoeba strains isolated from swimming pools in our previous research as well as standard Acanthamoeba castellanii (A. castellanii ) strains, were employed. The MCF-7 breast cancer cell line and the HaCaT human keratinocyte cell line served as models for assessing cytotoxic activity. Three Actinomycetes supernatants (20%) demonstrated strong inhibitory activity against all tested Acanthamoeba strains. MCF-7 cells were found to be more sensitive to one supernatant across all tested dilutions. These findings suggest that marine-derived Actinomycetes, are promising candidates for Acanthamoeba trophozoites. Moreover, the results may guide future research on cytotoxic bacterial metabolites produced by marine Actinomycetes.
https://ror.org/01jw2p796 
