The Journal of Poultry Science
Online ISSN : 1349-0486
Print ISSN : 1346-7395
ISSN-L : 1346-7395
Research Note
Construction and Analysis of Subtractive cDNA Library of Muscular Tissue in Hybrid of Chicken-Quail Related to Myogenesis
YaoWei LiangWei ZhenMiLa G.L. JiaerhengZongSheng ZhaoHongMei Zhang
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JOURNAL FREE ACCESS

2013 Volume 50 Issue 4 Pages 326-331

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Abstract
Myogenesis is a complex developmental progress in which a variety of transcription factors play essential roles in regulating myogenesis. However, the genetics programs that control myogenesis molecular mechanism are poorly understood. Therefore, meat-breeding and egg-breeding chicken were selected as male parent, Korean quails were used as female parent for hybridizing. To identify differentially expressed genes between the different hybrids, suppression subtractive hybridization (SSH) have been undertaken for generating cDNA collections of representative mRNAs specific to muscle tissue with meat-hybrid versus egg-hybrid. Following SSH, 54 clones were sequenced and analysed through BLASTX. The results showed that 26 Expressed Sequence Tags (ESTs) found no homology while the other 22 ESTs found the homology in GenBank and 4 ESTs might have correlation with myogenesis.
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© 2013 by Japan Poultry Science Association
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