抄録
In order to investigate the direct effects of thyroid hormones on steroidogenesis in avian ovary, quail granulosa cells were isolated from the three largest follicles and cultured with follicle stimulating hormone (FSH) at 41° Cin 5% CO2 and 95% air. After 72 hr of culture, the cells were incubated with luteinizing hormone (LH) for 3 hr and the progesterone contents in the media were measured by radioimmunoassay. Addition of thyroid stimulating hormone (TSH), thyroxine (T4), or triiodothyronine (T3) during 72 hr of culture with FSH or during 3 hr of incubation with LH showed no significant effects on progesterone production. These results suggested that TSH, T4, and T3 might not affect the steroidogenesis of quail granulosa cells.
