抄録
Early pregnancy factor (EPF) has been detected in pregnancy sera of many animal species. Isolation and purification of the EPF in pregnancy rat serum (Day 4) was attempted by using ammonium sulfate fractionation, ion-exchange chromatography, affinity chromatography and Immunoblotting. As a result, rat EPF was detected in 40% ammonium sulfate supernatant, CM-Sepharose Fast Flow 20 mM and 30 mM NaCl elution fraction, DEAE-Sepharose Fast Flow unbound fraction and Affinity chromatography unbound fraction. By the result of immunoblotting, there were three bands (22 KD, 24 KD and 28 KD) in the purified rat EPF fraction.
