妊馬血清剤処理によるウサギの過排卵に及ぼす黄体ホルモン前処理の効果について

抄録

An experiment was carried out to induce the superovulation in mature rabbits (Japanese White Breed, more than 2 kg in body weight) by PMS and CG with progesterone pretreatment. The principal method is as follows; 1mg progesterone twice-daily for five days, subsequently 20 I.U. PMS twice-daily for five days followed by CG and mating. After two days' intermission, does were autopsied and the number of ovulation point, blood follicle, and follicle in the ovary, recovery of ova from the tube and the fertility of ova recovered were examined.
The results in June demonstrated that the superovulation was successfully induced by the preceding combined treatment with a satisfactory recovery and fertility of the ova ; no significant differences were observed between the oestrous and dioestrous group, except remarkable occurrence of the blood follicle in the former. In October and November, when the rabbit was in moulting, and the reproductive activity was to be lower, a little retardation in the response to the treatment in the number of both positive case and ovulation point was found. The pseudopregnant does(10th and 15th, when the treatment started, without progesterone administration), also indicated somewhat inferior results with the wider range of ovulation point number, and particularly, in 10 day, none; but in 15th day, moderate number of fertilized ova were obtained.
In conclusion, it can be said that the superovulation in rabbits is able to produce by PMS accompanied with progesterone pretreatment of which no work has ever adopted, though successful results hitherto were demonstrated only by the pituitary extracts or preparations. There are probably some differences according to the season of year in the response. The full data will be presented elsewhere adding the same kind of experiments carried on in March.